| 鲁晓峰,杜国栋,孙海龙,徐树广.生物炭降低草莓根系线粒体膜功能伤害缓解乙草胺药害[J].中国南方果树,2022,51(3): |
| 生物炭降低草莓根系线粒体膜功能伤害缓解乙草胺药害 |
| Biochar alleviation effects on acetochlor phytotoxicity to mitochondrial membrane function in strawberry roots |
| 投稿时间：2021-07-06 修订日期：2021-11-18 |
| DOI：10.13938/j.issn.1007-1431.20210417 |
| 中文关键词: 生物炭 乙草胺 线粒体功能 保护性酶 |
| 英文关键词:biochar acetochlor strawberry function of the mitochondrial membrane protective enzyme |
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| 中文摘要: |
| 为探究除草剂乙草胺对草莓根系线粒体膜功能及保护酶系统活性的影响,明确土壤中施加生物炭对减缓乙草胺对草莓根系线粒体功能伤害的保护机制。以盆栽草莓‘宝交早生’(Fragaria × ananassa Duch.)为试材,设置除草剂乙草胺处理、乙草胺+生物炭处理及对照3个处理,结果表明,乙草胺处理导致根系线粒体膜透性增大,根系线粒体膜电位Δψm下降,根系活力和H+ - ATPase含量降低,O2-、H2O2产生速率持续升高,保护性酶SOD、POD活性持续增长,MDA含量不断增加。乙草胺能够破坏草莓根系线粒体膜结构,增加细胞质膜过氧化程度,造成线粒体膜功能下降。施加生物炭能有效缓解乙草胺对线粒体膜功能的伤害,提高草莓植株对乙草胺的适应能力。 |
| 英文摘要: |
| The aim of this study was to investigate the physiological mechanisms of root mitochondrial membrane structure and function and the activity of the protective enzyme system in strawberry roots in conjunction with the alleviation effects of biochar. The results would lay a theoretical foundation for the further study of the physiological mechanism of roots under herbicide injury during production. Seedlings of Fragaria × ananassa Duch. were used as materials. The experiment comprised three treatments: an acetochlor treatment, an acetochlor + biochar treatment and a control treatment. A potted experiment was conducted to determine how effectively biochar alleviated the effects of acetochlor on the mitochondrial membranes in strawberry roots and their antioxidant enzyme activities. Membrane permeability transition, O2- generation rate and the MDA content in strawberry roots clearly increased in response to treatment with the herbicide acetochlor. At the same time, mitochondrial membrane potential (Δψm) decreased, POD and SOD activities tended to increase continuously, and the MDA content (a root membrane peroxidation product) increased continuously. After biochar and acetochlor were applied to the soil, the effects of acetochlor on the mitochondrial membrane structure and function in strawberry roots were less severe. Acetochlor disrupted the mitochondrial membrane structure in strawberry roots and increased the degree of cytoplasmic membrane peroxidation, which ultimately reduced mitochondrial membrane function. The application of biochar to the soil could effectively alleviate the damage to mitochondrial membrane function in the roots of strawberry and could improve the adaptability of strawberry plants to acetochlor. |
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