| 楚宗丽,李亮杰,詹妞,陈娟娟,董向向,朱庆松.‘宁玉’草莓单茎尖培养技术研究[J].中国南方果树,2022,51(4): |
| ‘宁玉’草莓单茎尖培养技术研究 |
| Study on Single Stem Tip Culture of ‘Ningyu’ Strawberry |
| 投稿时间：2022-02-10 修订日期：2022-04-27 |
| DOI： |
| 中文关键词: ‘宁玉’草莓 单茎尖培养 培养基配方 |
| 英文关键词:‘Ningyu’ strawberry single stem tip tissue culture medium formulation |
| 基金项目:河南省科技攻关项目（222102110443），信阳农林学院青年教师科研基金项目（QN2021046），信阳农林学院高水平科研孵化器建设项目（FCL202007），信阳市草莓工程技术研究中心建设项目。 |
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| 中文摘要: |
| 草莓组培脱毒过程中可能会产生变异问题。本研究采用单茎尖离体培养的方法，筛选适宜‘宁玉’草莓初代培养、壮苗培养和生根培养的培养基配方。结果表明，适宜‘宁玉’草莓初代培养的培养基配方为：MS + 6-BA 0.20 mg·L-1 + NAA 0.05 mg·L-1，适宜壮苗培养的培养基配方为：MS + 6-BA 0.10 mg·L-1 + NAA 0.10 mg·L-1，适宜生根培养的培养基配方为：1/2 MS + NAA 0.10 mg·L-1，本研究为建立‘宁玉’草莓单茎尖培养技术体系提供技术支持。。 |
| 英文摘要: |
| In order to solve the variation problem in the process of culture in strawberry, we performed the single stem tip culture by screen the suitable medium of the primary culture, strong seedling culture and rooting culture of ‘Ningyu’ strawberry. The results showed that the suitable medium for the primary culture of ‘Ningyu’ strawberry was MS + 6-BA 0.20 mg·L-1 + NAA 0.05 mg·L-1; the suitable medium for the cultivation of strong seedling was MS + 6-BA 0.10 mg·L-1 + NAA 0.10 mg·L-1; the medium formula suitable for rooting culture was 1/2 MS + NAA 0.10 mg·L-1. This research provides technical support for the establishment of technology system for single stem tip culture of ‘Ningyu’ strawberry. |
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