| 刘毅杰,腾春凤,臧小平,井涛,赵维峰,云天艳.响应面优化法提高吸水链霉菌5-4菌株抗香蕉枯萎病的抑菌活性[J].中国南方果树,2023,52(4): |
| 响应面优化法提高吸水链霉菌5-4菌株抗香蕉枯萎病的抑菌活性 |
| Optimization of fermentation conditions of anti-Foc TR4 activity production in Streptomyces hygroscopicus sp. 5-4 by Response surface methodology |
| 投稿时间：2022-05-19 修订日期：2022-07-04 |
| DOI： |
| 中文关键词: 香蕉枯萎病 吸水链霉菌 抗真菌活性 响应面优化法 |
| 英文关键词:Banana fusarium wilt Streptomyces hygroscopicus antifungal active response surface methodology |
| 基金项目:本研究由中央级公益性科研院所基本科研业务费专项（1630092022004）；海南省自然科学基金高层次人才项目（321QN0913, 321RC543, 320RC685）资助。 |
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| 中文摘要: |
| 为提高吸水链霉菌5-4的抑菌活性，本研究以拮抗Foc TR4病菌的抑菌率为响应值，采用响应面三步法对链霉菌5-4的发酵条件进行优化。大幅度提高了链霉菌5-4发酵提取物对香蕉枯萎病4号生理小种（Foc TR4）的抗真菌活性，其中可溶性淀粉（21.512 g/L）、氯化钠（4.734 g/L）、接种量（3.217%）是次级代谢物抗真菌活性高的关键因素，优化后的抑菌活性达到了88.36%，比优化前（54.96%）提高了33.4%。优化后发酵提取物（500 μg/mL）的校正孢子萌发抑制率达到100%，比优化前提高了36.38%，且经过提取物处理后Foc TR4病菌的菌丝出现明显的分叉增生、肿大等现象。链霉菌5-4发酵条件的优化可以为以后分离抑菌活性物质提供条件，也为后续菌株工业化生产奠定了基础。 |
| 英文摘要: |
| In order to improve the antifungal activity of Streptomyces hygroscopicus sp. 5-4, the response surface methodology was used to optimize the fermentation conditions of Streptomyces hygroscopicus sp. 5-4. The antifungal activity of Streptomyces hygroscopicus sp. 5-4 fermentation extract on Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) were significantly improved by optimization of fermentation conditions, of which soluble starch (21.512 g/L), NaCl (4.734 g/L), and inoculum amount (3.217%) were the key factors for the high antifungal activity were optimized. The antifungal activity of optimized fermented extract was 88.36%, which was 33.4% higher than before optimization (54.96%). The corrected spore germination inhibition rate of the optimized fermented extract (500 μg/mL) was 100%, which was 36.38% higher than the before optimization. The mycelia of Foc TR4 were showed obvious branching hyperplasia and swelling after treated with the extracts. The optimization of the fermentation conditions of Streptomyces hygroscopicus sp. 5-4 can provide conditions for the separation of antifungal active substances in the future, and also lay a foundation for the industrial production of subsequent strains. |
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