王晓宇,徐冬英,王彤彤,卢艳春,谭德锦,杨志强,蒋婷.油梨突脐蠕孢叶斑病病原菌鉴定及室内防治药剂筛选[J].中国南方果树,2023,52(5): |
油梨突脐蠕孢叶斑病病原菌鉴定及室内防治药剂筛选 |
Idientification of Exserohilum Leaf Spot on Avocado (Persea americana) and Control Fungicides Screening |
投稿时间:2022-08-18 修订日期:2022-09-21 |
DOI: |
中文关键词: 油梨 叶斑病 嘴突脐蠕孢 杀菌剂 |
英文关键词:Avocado (Persea americana) Leaf spot Exserohilum rostratum Fungicides |
基金项目:广西农科院稳定资助科研团队项目(桂农科2021YT168);广西农科院科技发展基金项目(桂农科2020ZX11);广西农业科学院基本科研业务专项项目(桂农科2022YM27) |
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中文摘要: |
【目的】为明确在广西崇左市油梨种植基地发现的一种叶斑病的病原菌及其生物学特性,以及该病害的防治药剂。【方法】通过形态学鉴定及分子生物学方法,对该病原菌进行鉴定,并对该病原菌进行了生物学特性测定及防治杀菌剂的室内筛选。【结果】该叶斑病的病原菌为嘴突脐蠕孢(Exserohilum rostratum),该病原菌的最适生长温度为28℃,最适培养基为PDA培养基,最适pH为7,黑暗条件有利于该菌的生长。参试的250g/L吡唑醚菌酯乳油、10%苯醚甲环唑水分散粒剂、40%百菌清悬浮剂、430g/L戊唑醇悬浮剂和40%腈菌唑可湿性粉剂对嘴突脐蠕孢均有抑制效果,其中10%苯醚甲环唑水分散粒剂、430g/L戊唑醇悬浮剂和40%腈菌唑可湿性粉剂对该菌生长的抑制效果较好,其EC50分别为0.0163mg/L、0.0420mg/L、0.1109mg/L。 |
英文摘要: |
[Purpose]In order to identify the pathogen of a new leaf spot on avocado (P. americana), the samples were collected from two avocado plantations in Chongzuo, Guangxi. [Method]The species of the pathogen was identified by morphological identification and molecular dection. The biological characteristics of the pathogen and the in vitro screening of fungicides for controlling this pathogen were studied. [Result]The results showed that the pathogen was Exserohilum rostratum, the optimal growth temperature of the pathogen was 28°C, the optimal medium was PDA, and the optimal pH was 7. The dark conditions were favorable for the growth of the pathogen. The 250g/L pyraclostrobin emulsifiable concentrate, 10% difenoconazole water dispersible granule, 40% chlorothalonil suspending concentrate, 430g/L tebuconazole suspending concentrate and 40% myclobutanil wettable powder tested had the inhibitory effect on the pathogen, among which 10% difenoconazole water dispersible granule, 430g/L tebuconazole suspending concentrate and 40% myclobutanil wettable powder had the best inhibitory effect on the growth of the pathogen, and their EC50 were 0.0163mg/L, 0.0420mg/L and 0.1109mg/L. |
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