| 应支萍,李彪,张澳,王瑞丽,熊智,施蕊.不同发育时期澳洲坚果果皮抗氧化物质成分分析[J].中国南方果树,2023,52(6): |
| 不同发育时期澳洲坚果果皮抗氧化物质成分分析 |
| Analysis of antioxidant composition of macadamia pericarp at different developmental stages |
| 投稿时间：2022-08-21 修订日期：2022-09-11 |
| DOI： |
| 中文关键词: 坚果果皮 发育时期 酚酸类 代谢组学 药用价值 |
| 英文关键词:Macadamia pericarp Developmental periods Phenolic acids Metabolomics Medicinal value |
| 基金项目:国家重点研发计划项目“林下中药材优质生产关键技术研究与集成示范”（2021YFD1000202）；国家现代农业产业技术体系（CARS-21-05B）；云南省重大科技专项（202102AE090042，202002AA10007，2019ZG00901）；云南省千人计划高端外国专家专项（2019013）；云南省级财政林业科技推广示范专项〔2020〕ts09号；外专引智项目（G2021039002，202105AQ130011）；昆明市科技揭榜制项目（2021JH002） |
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| 中文摘要: |
| 以云南省永德县大雪山乡澳洲坚果种植基地广泛种植的优良品种O.C.为研究材料，利用超高效液相色谱-串联质谱（UPLC-MS/MS）的技术方法，对该品种花后30天、60天和90天的果皮进行代谢组学分析，探究不同发育时期澳洲坚果果皮酚酸类物质组分及含量的变化，为果皮中酚酸类抗氧化物质的开发和利用提供参考依据。结果表明：不同发育时期果皮中的酚酸类物质组分及含量有较大差异，在检测到的114个代谢物中，绿原酸 (3-O-咖啡酰奎宁酸)、1-O-阿魏酰-D-葡萄糖和1-O-对香豆酰-β-D-葡萄糖等物质的含量在花后30天最高，表明发育前期需要大量能量物质作为生长代谢原料；熊果苷、6'-咖啡酰熊果苷、红景天苷、龙胆酸、阿魏酸、3,4-二没食子酰莽草酸、没食子苯乙酮、原儿茶酸、水杨酸以及豆腐果苷等多种物质在花后60天及90天含量较高；6'-对香豆酰熊果苷和3,4-二甲氧基肉桂酸为花后90天标志性物质。结论： 本文分析测试了澳洲坚果果实不同发育期果皮中酚酸类物质的种类及含量变化，发现果皮中含有美白、抗菌等活性成分，具有一定的药用价值，为今后澳洲坚果早期落果及采后果皮加工利用、保健品及药物开发等奠定了很好的基础。 |
| 英文摘要: |
| Using the excellent variety O.C., which is widely grown in the macadamia plantation in Daxueshan Township, Yongde County, Yunnan Province, as the research material, the metabolomic analysis of the pericarp of this variety at 30, 60 and 90 days after flowering was performed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to investigate the changes in the phenolic acid components and contents of macadamia pericarp at different developmental periods, and to provide a reference basis for the development and utilization of phenolic acid antioxidants in the pericarp. The results showed that the phenolic acid content of macadamia pericarp during different developmental periods was investigated. Among the 114 metabolites detected, the contents of chlorogenic acid (3-O-caffeoylquinic acid), 1-O-feruloyl-D-glucose and 1-O-p-coumaroyl-β-D-glucose were the highest at 30 days after flowering, indicating that a large amount of energy is required as a raw material for growth metabolism in the early stage of development. Arbutin, 6'-caffeoylarbutin, rhodiogenin, gentianic acid, ferulic acid, 3,4-digallocatechuic acid, gallocatechuic acetophenone, protocatechuic acid, salicylic acid and bean curd glucoside were high at 60 and 90 days after flowering; 6'-p-coumaroylarbutin and 3,4-dimethoxycinnamic acid were the landmark substances at 90 days after flowering. Conclusion: In this paper, we analyzed and tested the types and content changes of phenolic acids in the peel of macadamia nuts at different developmental stages, and found that the peel contains whitening, antibacterial and other active ingredients with certain medicinal value, which laid a good foundation for future processing and utilization of the peel of macadamia nuts after early fruit drop and harvesting, health products and drug development. |
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