| 陈思宇,柳凤.杧果MiNPR1抗病基因克隆及生物信息学分析[J].中国南方果树,2023,52(4): |
| 杧果MiNPR1抗病基因克隆及生物信息学分析 |
| Cloning and Bioinformatics Analysis of Mangifera indica Disease-resistant Gene MiNPR1 |
| 投稿时间：2022-09-23 修订日期：2022-11-08 |
| DOI：10.13938/j.issn.1007-1431.20220580 |
| 中文关键词: MiNPR1 杧果 基因克隆 生物信息学 |
| 英文关键词:MiNPR1 Mangifera indica Gene cloning Bioinformatics |
| 基金项目:海南省重点研发计划（ZDYF2021XDNY158）；海南省自然科学基金（320QN319）；中国热带农业科学院基本科研业务费专项资金（1630062022014） |
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| 中文摘要: |
| NPR1是SA介导的植物抗病通路中的转录共激活因子。本实验采用RT-PCR技术从杧果（Mangifera indica）中分离得到了MiNPR1-1和MiNPR1-2。序列分析显示：MiNPR1-1基因编码471个氨基酸，MiNPR1-2基因编码445个氨基酸。2种MiNPR1蛋白皆含BTB/POZ结构域和锚蛋白重复序列，非跨膜蛋白且无信号肽，为不稳定酸性亲水蛋白。蛋白结构预测表明：MiNPR1蛋白由α-螺旋、无规则卷曲、延伸链和β-折叠构成，三级结构与二级结构相符。进化关系得出：MiNPR1-1为单独分支起源，MiNPR1-2蛋白与阿月浑子（Pistacia vera）的XP_031280798.1聚类为一簇。本研究克隆了杧果NPR1基因，预测其编码蛋白特征，为后续研究该基因在杧果上的功能及培育杧果优良抗病品种提供参考。 |
| 英文摘要: |
| NPR1 gene is a transcriptional co-activator in SA-mediated plant disease-resistant pathway. In this study, MiNPR1-1 and MiNPR1-2 were cloned and isolated from Mangifera indica by RT-PCR. MiNPR1-1 gene encodes 471 amino acids, and MiNPR1-2 gene encodes 445 amino acids. Both MiNPR1 proteins contain BTB/POZ domain and ankyrin repeats. MiNPR1-1 and MiNPR1-2 are unstable acidic hydrophilic proteins without transmembrane domains and signal peptides. MiNPR1 proteins are composed of four conformations: α-helix, random coil, extended strand and β-turn. The tertiary structure is consistent with the secondary structure. MiNPR1-1 is a single branch origin, while MiNPR1-2 protein is clustered with XP_031280798.1 of Pistacia vera. In this study, the mango NPR1 genes were cloned, and the characteristics of the protein encoded by MiNPR1 were predicted, which provided a reference for the follow-up study of the function of this gene in mango and the cultivation of excellent disease-resistant varieties of mango. |
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