| 张曼莹,文晓鹏,乔光,杜倩,李蕊蕊,闫艺心,洪怡.‘玛瑙红’樱桃茎尖培养脱植原体快繁体系建立及优化[J].中国南方果树,2023,52(4): |
| ‘玛瑙红’樱桃茎尖培养脱植原体快繁体系建立及优化 |
| Establishment and optimization of rapid propagation system for the elimination of phytoplasma from‘Ma Naohong’cherry by stem tip culture |
| 投稿时间：2022-10-20 修订日期：2022-10-20 |
| DOI：10.13938/j.issn.1007-1431.20220633 |
| 中文关键词: 快繁 植原体 茎尖培养 热处理 |
| 英文关键词:Gradingrapid propagation Phytoplasma Sem tip culture Heat treatment |
| 基金项目:贵州省科技厅项目“贵州樱桃植原体病害遗传多样性研究及无病苗培育”（黔科合支撑[ 2020 ]1Y133号） |
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| 中文摘要: |
| 为了解决玛瑙红樱桃植原体病害问题，以玛瑙红樱桃休眠芽为外植体，优化了玛瑙红茎尖快繁体系并对该体系进行ISSR检测，同时采用茎尖培养法、热处理结合茎尖二次培养法对玛瑙红樱桃植原体进行脱除。结果如下：茎尖萌发培养基为MS+6-BA 1.0 mg/L+IBA 0.2 mg/L+TDZ 0.3 mg/L，萌发率为80.67 %；增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.1 mg/L+GA3 1.5 mg/L，平均增殖系数为5.07；生根培养基为MS+IBA 0.5 mg/L+NAA 0.3 mg/L，生根率为79.17 %；ISSR检测未发生变异，表明该快繁体系稳定；最佳茎尖培养脱除方法为：取休眠芽茎尖0.3~0.6 mm进行培养，脱除率为66.67 %，成活率为52.22 %，最佳热处理结合茎尖培养脱除方法为：组培苗经38 ℃处理21天后剥取0.3~0.6 mm茎尖培养，脱除率为86.67 %，成活率为42.22 %。 |
| 英文摘要: |
| In order to solve the problem of Prunus pseudocerasu ‘Ma Naohong’ phytoplasma disease, the P. pseudocerasu ‘Ma Naohong’ dormant buds were taken as explants to optimize the rapid propagation system of P. pseudocerasu ‘Ma Naohong’ stem tip, and the system was tested by ISSR. Meanwhile, the stem tip culture and heat treatment combined with the stem tip secondary culture were also used to eliminate the P. pseudocerasu ‘Ma Naohong’ phytoplasma. The results showed that the medium for stem tip germination was MS + 6-BA 1.0 mg/L + IBA 0.2 mg/L + TDZ 0.3 mg/L, and the germination rate was 80.67 %. The proliferation medium was MS + 6-BA 1.0 mg/L + IBA 0.1 mg/L + GA3 1.5 mg/L, and the average proliferation coefficient was 5.07. The rooting medium was MS + IBA 0.5 mg/L + NAA 0.3 mg/L, and the rooting rate was 79.17 %. No variation occurred in the ISSR assay, indicating that the rapid propagation system was stable. The best method for stem tip culture to eliminate phytoplasma was to strip 0.3~0.6 mm of dormant bud stem tip for culture. The elimination rate was 66.67 % and the survival rate was 52.22%. The best heat treatment coupled with stem tip culture elimination method:Tissue culture seedlings were treated at 38 ℃ for 21 days and then stripped to 0.3~0.6 mm stem tips for culture，with 86.67 % elimination rate and 42.22 % survival rate. |
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