| 李蕊蕊,吴茂宏,任菲宏,王贵,张曼莹,闫艺心,乔光.沙子空心李离体快繁及遗传变异检测[J].中国南方果树,2023,52(6): |
| 沙子空心李离体快繁及遗传变异检测 |
| Culture of Prunus salicina Lindl. cv ‘Shazikongxinli’ in vitro and Assessments of Its Genetic Variation |
| 投稿时间：2022-11-08 修订日期：2022-11-30 |
| DOI：10.13938/j.issn.1007-1431.20220662 |
| 中文关键词: 沙子空心李 离体培养 分子标记 遗传变异 |
| 英文关键词:Prunus salicina Lindl. cv ‘Shazikongxinli’ clture in vitro molecular markers genetic variation |
| 基金项目:贵州省科技支撑计划项目(黔科合支撑[2020]1Y023)；铜仁市沙子空心李工程技术研究中心创新能力建设(黔科合服企[2019]4013) |
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| 中文摘要: |
| 为了贵州省沿河县沙子空心李资源的提纯复壮及优质苗木的工厂化繁育,本研究以沙子空心李冬季休眠芽为外植体,通过设置不同激素质量浓度的处理,探索最佳增殖和生根培养基条件,并利用SSR及ISSR两种分子标记,对空心李组培苗遗传变异进行检测。结果表明：组培苗在增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+GA3 0.5 mg/L+KT 1.5 mg/L时,增殖系数较高为5.15,且增殖芽健壮；在生根培养基为1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L时,生根率最高为65%,平均生根数为9.35,且主根粗壮须根极多；分别用21条ISSR引物和22对SSR引物对增殖苗进行遗传变异检测,结果表明在引物检测范围内沙子空心李离体培养至第5代仍然保持其遗传稳定性,该离体快繁体系可用于工厂化育苗生产。 |
| 英文摘要: |
| To purify and enhance Prunus salicina Lindl. cv‘Shazikongxinli’ sources and the large-scale breeding of high-quality seedlings, with the winter dormant buds of ‘Shazikongxinli’ as explants, an orthogonal experiments were conducted, in which different concentrations of plant regulators were combined on the MS media. In the results, a highly efficient micropropagation system was established for this cultivar. The genetic variation was examined using two molecular markers, SSR and ISSR. It was shown that in a culture medium with MS + 6-BA 0.5 mg/L + NAA 0.1 mg/L + GA3 0.5 mg/L + KT 1.5 mg/L, the proliferation coefficient was 5.15 and the proliferation bud was robust. When the rooting medium was 1/2MS + IBA 0.5 mg/L + NAA 0.2 mg/L, the highest rate of root growth was 65%, the average number of roots was 9.35, and the main root was very large. Genetic variants were tested with ISSR and SSR molecular markers, and within the detection range of the 21 primers for ISSR and 22 pairs primers for SSR, ‘Shazikongxinli’ was cultured in vitro until its stable heritability in the fifth generation. The in vitro rapid propagation system of ‘Shazikongxinli’ developed in this study can be used for industrial seedling production. |
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