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闫素云,周先艳,王自然,杜玉霞,李晶,钟文彬,付小猛,岳建强.微生物菌化肥互作对柠檬苗生长、土壤理化性质及酶活性的影响[J].中国南方果树,2024,53(3):
微生物菌化肥互作对柠檬苗生长、土壤理化性质及酶活性的影响
Effects of microbial bacterial fertilizer interaction on the growth of lemon seedlings, soil physical and chemical properties and enzyme activity
投稿时间:2022-12-29  修订日期:2023-03-21
DOI:
中文关键词:  柠檬  微生物菌  化肥  生长状况  土壤养分
英文关键词:Lemon  Microbial bacteria  Fertilizer  Growth status  Soil nutrients
基金项目:国家现代农业(柑橘)产业技术体系建设专项(CARS-26);云南省重大科技专项计划(202102AE090054,202102AE090020);云南省科技人才与平台计划(202105AD160049);国家科技部国家重点研发计划(2021YFD1100406)资助。第一作者闫素云,(1996-)女,硕士,研究实习员,主要从事土壤肥力与根际微生物。E-mailyansuyun979@163.com。*通信作者岳建强,(1967-)男,硕士,研究员,主要从事热带亚热带果树资源与育种研究。E-mailyjq7009@163.com。 *
作者单位E-mail
闫素云 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 18787822509@163.com 
周先艳 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 710925568@qq.com 
王自然 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 wangziran2004@163.com 
杜玉霞 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 duyuxia117@163.com 
李晶 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 419253166@qq.com 
钟文彬 云南大学 资源植物研究院 13870560387@163.com 
付小猛 云南省农业科学院热带亚热带经济作物研究所 云南 保山 meng860506@126.com 
岳建强* 云南省农业科学院热带亚热带经济作物研究所 云南 瑞丽 yjq7009@163.com 
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中文摘要:
      研究等量化肥条件下配施四种不同浓度的微生物菌对柠檬苗树体生长、土壤矿质、酶活性的影响,为柠檬的绿色高效种植提供技术支撑。以‘云柠1号’为试验材料,在云南瑞丽进行了1年的盆栽试验。设置丛枝菌根-化肥、拜赖青霉-化肥、枯草芽孢杆菌-化肥、酵母菌-化肥处理,每个处理设置4个浓度梯度,以单施化肥作为对照。在12次施肥结束后,测量植株地上部分生物量和取土样分析土壤理化指标和酶活性。与单施化肥的处理相比较,微生物菌能够提高肥料利用率,调节土壤酶活性,进而提高并较好的保持柠檬植株生长。综合效果来看,拜赖青霉-化肥效果最佳。其中,在BT2(62.5ml/株+20g/株化肥)处理下对植株株高、南北方向树冠幅、叶绿素含量、土壤中Zn、Ca、Mg含量、土壤脲酶活性这七项指标有显著增加;在BT3(83.3ml/株+20g/株化肥)处理下对植株茎粗、冠幅、结果枝叶面积、土壤中Fe、Mn含量五项指标得到显著提升;在 BT4(125ml/株+20g/株化肥)浓度下增加了营养枝叶面积、提高了土壤有机质含量、土壤中N、P、K、Cu含量及土壤中性磷酸酶活性。相对于单施化肥而言,拜赖青霉-化肥能够提高化肥的利用率,增强土壤肥力,而该研究结果也可为合理利用微生物菌配施化肥来提高柠檬植株生长和改善土壤养分提供理论依据。
英文摘要:
      Studied the effects of four different concentrations of microbial agents on the growth of lemon seedlings, soil mineral and enzyme activities under the condition of equal amount of chemical fertilizer, it provides technical support for green and efficient planting of lemon. "Yunning No. 1" was used as test material, the pot experiment was carried out in Ruili, Yunnan province for one year. Set arbuscular mycorrhizae-fertilizer, penicillium baileyi-fertilizer, bacillus subtilis-fertilizer, yeast-fertilizer treatment, four concentration gradients were set for each treatment, with single application of chemical fertilizer was used as a control. After 12 times of fertilization, the aboveground biomass of plants was measured and soil samples were taken to analyze soil physical and chemical indicators and enzyme activities. Compared with the treatment of applying chemical fertilizer alone, microbial bacteria can improve the utilization rate of fertilizers, regulate the activity of soil enzymes, and then improve and better maintain the growth of lemon plants. In terms of comprehensive effect, the combination of penicillium baileyi and chemical fertilizer has the best effect. Among them, under the treatment of BT2 (62.5ml/plant + 20g/plant chemical fertilizer), the seven indexes of plant height, tree crown width in north-south direction, chlorophyll content, Zn, Ca, Mg content in soil, and soil urease activity were significantly increased; Under the treatment of BT3 (83.3ml/plant +20g/plant chemical fertilizer), the five indexes of plant stem diameter, crown width, bearing branch and leaf area, and the content of Fe and Mn in soil were significantly increased; At the concentration of BT4 (125ml/plant + 20g/plant chemical fertilizer), the area of vegetative branches and leaves was increased, the content of soil organic matter, the content of N, P, K, and Cu in the soil and the activity of neutral phosphatase in the soil. Compared with single application of chemical fertilizer, combined application of chemical fertilizer with penicillium baileyi can increase the utilization rate of chemical fertilizer and enhance soil fertility. The results of this study can also provide a theoretical basis for the rational use of microbial bacteria combined with chemical fertilizer to improve the growth of lemon plants and improve soil nutrients.
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