| 於浩然,吴婧波,詹儒林,柳凤,姚全胜,李国平,魏卿.绿色荧光蛋白（GFP）标记芒果细菌性角斑病病原菌及其定殖规律[J].中国南方果树,2024,53(4): |
| 绿色荧光蛋白（GFP）标记芒果细菌性角斑病病原菌及其定殖规律 |
| Colonization Rule of GFP-tagged Xanthomonas citri pv. mangiferaeindicae in Different Tissues of Mango |
| 投稿时间：2023-03-09 修订日期：2023-04-27 |
| DOI： |
| 中文关键词: 芒果细菌性角斑病 野油菜黄单胞菌芒果致病变种 GFP标记 定殖规律 |
| 英文关键词:Mango bacterial black spot Xanthomonas citri pv. Mangiferaeindicae GFP-labeled Colonization |
| 基金项目:1、云南省科技厅科技计划项目（202104BI090012）；2、三亚亚州湾科技城科技专项资助，项目编号SCKJ-JYRC-2022-60；3、广东省重点领域研发计划(2022B0202070002) |
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| 中文摘要: |
| 为研究芒果细菌性角斑病病菌在不同芒果组织中的定殖规律。利用电击转化法将质粒pBBR1MCS2-Tac-EGFP导入野油菜黄单胞菌芒果致病变种（Xanthomonas citri pv. mangiferaeindicae）Xcm003中，通过转化子生长表型和外源基因的PCR鉴定，成功获得带绿色荧光（GFP）标记的转化子Xcm003-EGFP，采用室内刺伤和盆栽苗灌根接种试验，结合组织学观察法，追踪该病原菌在不同芒果组织中的定殖规律。试验表明：病原菌通过伤口先侵入芒果果实外表皮细胞层，随着侵染时间推移，向内层果皮细胞层垂直扩散并定殖，后期，病原菌在伤口处大量聚集，出现“隆起”状病斑；在叶片中，病原菌从伤口侵入上表皮细胞层，随后迁移到叶肉细胞间隙，侵染后期，病原菌在气孔和伤口定殖，造成气孔和伤口堵塞，导致接种点出现黑褐色“水渍”病斑；盆栽苗灌根实验发现，该病原菌可在土壤中定殖，入侵芒果根部，但并不扩散至其他芒果组织，不会危害整株幼苗。研究表明，芒果细菌性角斑病病菌可在不同芒果组织中定殖，但仅表现为局部侵染特性。本研究初步探索了芒果细菌性角斑病病菌在不同芒果组织中的定殖规律，为探究该病原菌与寄主互作机制及其流行防控提供理论依据。 |
| 英文摘要: |
| For the purpose of exploring the colonization of Xanthomonas citri pv. mangiferaeindicae strains in different tissues of mango, plasmid pBBR1MCS2-Tac-EGFP carrying GFP gene was successfully transferred into the pathogen by using the electro-transformation method. The infection histological process in different tissues of mango was observed by using transformants labeled with GFP. The inoculation test showed that the bacteria first invaded the epicarp cell layer of mango fruit through the wound, and then spread vertically to the endocarp cell layer and colonized with the passage of time. In the late stage of inoculation, the pathogen multiplied in the wound and appeared to ‘bulge’ lesions. In the leaves, the bacteria invaded the epidermis cell layer of mango leaf from the wound, then transferred to the mesophyll cell space，and eventually colonized the stomata and wounds. Black-brown ‘water stain’ disease speckle appeared at the inoculation site. Root-irrigation experiments showed that the pathogen colonizes in soil and invades the root of mango but not spread to other mango tissues. The results showed that the pathogen could colonize different mango tissues, but it only showed characteristics of local infection. This study preliminarily explored the colonization of mango bacterial black spot in different mango tissues. The above findings provide theoretical basis for exploring the interaction mechanism between the pathogen and the host and its epidemic control. |
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