| 刘政,易善萍,李谢雨,杨 立,程寅胜,聂显双,伍 涛.梨树冠层光响应基因PpPRR5b的克隆与功能分析[J].中国南方果树,2023,52(6): |
| 梨树冠层光响应基因PpPRR5b的克隆与功能分析 |
| Cloning and functional analysis of PpPRR5b gene related to canopy light responses in pear |
| 投稿时间：2023-03-17 修订日期：2023-03-17 |
| DOI： |
| 中文关键词: 梨树 PpPRR5b 基因克隆 功能分析 冠层光响应 |
| 英文关键词:Pear PpPRR5b Gene cloning Functional analysis Canopy light responses |
| 基金项目:现代农业(梨)产业技术体系(CARS-28)、湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-05)和湖北省农业科学院第二批青年拔尖人才培养计划 |
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| 中文摘要: |
| 连锁的转录-翻译反馈路径在调控生物钟输出途径中起重要作用,而伪应答调控蛋白PRR则是其关键的遗传组分。尽管PRR基因在非生物反应、细胞延伸和光周期开花反应等方面的调控作用已被证实,但它们在其它光相关的农艺性状(如光合作用)中的作用还有待进一步阐明。本研究从梨叶组织中克隆了一个拟南芥AtPRR5的同源基因,命名为PpPRR5b。该基因全长2019 bp,共编码672个氨基酸。PpPRR5b蛋白相对分子量为74.23kD,理论等电点为6.28,定位于细胞核,具有亲水性。二级结构预测结果显示,PpPRR5b主要以无规则卷曲为主(占60.01%)。烟草叶片瞬时表达结果表明,随着红光强度的增强,超量表达PpPRR5b基因的叶组织能显著抑制净光合速率、气孔导度及胞间CO2浓度水平。本研究初步证实PpPRR5b具有响应光信号,抑制光合作用的功能。 |
| 英文摘要: |
| PRRs (Pseudo-Response Regulator) are key genetic components of interconnected transcriptional-translational feedback that regulate circadian clock-output pathways. Although PRR genes are well-established regulators of abiotic responses, cell elongation and photoperiodic flowering responses, their regulatory roles in other light-dependent agricultural traits, including photosynthesis, merit further exploration. Here, we cloned one ortholog of AtPRR5 in Arabidopsis (hereafter referred to as PpPRR5b) from pear leaves. A 2019-bp full-length sequence of PpPRR5b encoded 672 amino acids with a calculated molecular mass of 74.23kD and a pI of 6.28. PpPRR5b was a hydrophilic protein located in the nucleus. The secondary structure of protein was mainly consisted of random coil (accounted for 60.01%). Significantly reduced level of the photosynthetic parameters, i.e., net photosynthetic rate, stomatal conductance and internal CO2, were observed in PpPRR5b overexpression N. benthamiana leaves under enhanced red light signals. All the results presented in this study indicated that PpPRR5b was associated with negatively regulating photosynthetic performance in response to varying light signals. |
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