| 陈华蕊,何书强,李洪立,党志国,朱敏,冯振翠,杨子琴,陈业渊.红皮红肉火龙果组培快繁技术的研究[J].中国南方果树,2023,52(6): |
| 红皮红肉火龙果组培快繁技术的研究 |
| Tissue culture and rapid propagation technology for Hylocereu polyrhizus |
| 投稿时间：2023-04-01 修订日期：2023-05-04 |
| DOI： |
| 中文关键词: 火龙果 不定芽诱导 诱导率 组培快繁 |
| 英文关键词:Hylocereus polyrhizus Adventitious bud induction Induction rate Induction Callus tissue culture and Rapid propagation |
| 基金项目:海南省基础与应用基础研究计划(自然科学领域)高层次人才项目资助项目“火龙果胚性愈伤组织的诱导及再生体系的建立”(编号：2019RC311) |
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| 中文摘要: |
| 为建立火龙果的组培快繁体系，以红皮红肉火龙果(Hylocereu polyrhizus) 的幼嫩茎段为外植体进行组培快繁研究，结果表明，最适的外植体消毒的方法为75% 酒精消毒20 s，2%次氯酸钠5min， 0.1% 升汞10 min，污染率为11.11% ，成活率达73.33%。MS+6-BA 3.0 mg/L + NAA 0.2mg/L为诱导不定芽效果最好的培养基，诱导率83.33%。添加6-BA6.0 mg/L 和NAA0.1 mg/L有利于芽的增殖和分化，增殖系数可达5.9。研究发现最佳的壮苗培养基为MS+6-BA2.0 mg/L +NAA0.1 mg/,虽然繁殖系数不高，但其不定芽生长粗壮。最适宜的生根培养基为：MS+IBA 0.6 mg/L+NAA0.2 mg/L。，生根率达到 100% ，平均生根数6.83条每株。 |
| 英文摘要: |
| In order to establish the system of tissue culture and rapid propagation of Hylocereus polyrhizus, the young stem segments of hylocereus polyrhizus were used as explants for tissue culture and rapid propagation. The results showed that the survival rate of seedlings would reach to73.33% with pollution rate at 11.11% if soaked in 75% ethanol for 20s，2% sodium hypochlorite for 5 min，and 0.1% mercuric chloride for 10 min successively.the best medium for inducing adventitious buds was MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L, the induction rate of adventitious buds was 83.0%. Addition of 6-Ba 6.0 mg/L and NAA 0.1 mg/L was beneficial to bud proliferation and differentiation. The proliferative coefficient of adventitious shoots was as high as5.9;. the optimal medium for strong seedling growth was MS + 6-ba 2.0 mg/l + NAA 0.1 mg/l. the propagation coefficient of adventitious buds was low, but the adventitious buds grew well. The optimal rooting medium was MS + IBA 0.6 mg/L + NAA 0.2 mg/L. The rooting rate was 100% and the average root number was 6.83 per plant. |
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