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郭慧慧,林丛发,蒋元斌,徐绍翔.软枣猕猴桃‘奇异莓6号’离体再生技术[J].中国南方果树,2024,53(1):
软枣猕猴桃‘奇异莓6号’离体再生技术
In Vitro Regeneration Technology for Actinidia arguta ‘Qiyimei No.6’
投稿时间:2023-05-02  修订日期:2023-06-04
DOI:10.13938/j.issn.1007-1431.20230208
中文关键词:  软枣猕猴桃  离体培养  无芽茎段  高效再生
英文关键词:Actinidia arguta  in vitro culture  non-bud stem segment  efficient regeneration
基金项目:
作者单位E-mail
郭慧慧* 宁德市农业科学研究所 821348920@qq.com 
林丛发 宁德市农业科学研究所 lcf877@126.com 
蒋元斌 宁德市农业科学研究所 abcjyb@126.com 
徐绍翔 宁德市农业科学研究所 1598693892@qq.com 
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中文摘要:
      以软枣猕猴桃无芽茎段为外植体进行离体培养,具有成本低、取材方便、材料充足等优点,但以往的研究表明无芽茎段诱导再生率低。本试验以‘奇异莓6号’软枣猕猴桃无芽茎段为外植体,探究不同植物生长调节剂、光照强度、温度、苗龄对不定芽诱导的影响。结果表明:最佳外植体为苗龄30d的无芽茎段,在光照12h/d(光照强度50LX)、室温20℃下,不定芽诱导的最适培养基为MS+2mg/L ZT,不定芽以间接途径发生,培养40d无芽茎段两端开始形成淡绿色愈伤组织,培养45d愈伤组织表面出现红色小点,开始形成不定芽,培养55d愈伤组织诱导率、愈伤组织分化率及不定芽数均到最大,分别为100%、100%及18.87个/块。待苗长至3cm左右时转到MS+0.4mg/L IBA中进行生根培养,培养30d生根率可达100%、根数为8.53/株、根长1.68cm、根粗0.14cm。
英文摘要:
      Using the non-bud stem segment as explants in vitro culture,it has the advantages of low cost,convenient materials,and abundant test materials. However, previous studies have shown that the induced regeneration rate of non-bud stem is extremely low. In this experiment, the non-bud stem segment of Actinidia arguta ‘Qiyimei No.6’ was used as explants to explore the effects of different plant growth regulators, light intensity, temperature and seedling age on adventitious bud induction. The results showed that the best explants were the non-bud stem segment with the seedling age of 30 days. The photoperoid is 12h/d(light intensity 50LX). The room temperature is 20℃. The best medium for adventitious buds induction of Actinidia arguta was MS+2 mg/L ZT. The adventitious buds occurred in an indirect way. After 40 days of culture, light green callus began to form at both ends of the non-bud stem segment. On the 45th day, red spots appeared on the surface of callus and began to form adventitious buds. The callus induction rate, callus differentiation rate and the number of adventitious buds reached the maximum at 55 days, which were 100%, 100% and 18.87 / piece, respectively. When the seedling grows to about 3cm, it was transferred to MS + 0.4 mg/L IBA for rooting culture. After 30 days of culture, the rooting rate was 100%, the number of roots was 8.53/plant, the root length was 1.68cm and the root diameter was 0.14cm.
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