| 何利钦,王丽华,庄启国,张 瑶,王凤琪,张 茜.不同猕猴桃的花粉形态观察与分析[J].中国南方果树,2024,53(4): |
| 不同猕猴桃的花粉形态观察与分析 |
| Observation and Analysis of Pollens Morphology of Different Kiwifruit |
| 投稿时间：2023-06-19 修订日期：2023-10-09 |
| DOI：10.13938/j.issn.1007-1431.20230290 |
| 中文关键词: 猕猴桃 花粉形态 扫描电镜 聚类分析 |
| 英文关键词:Kiwifruit, Pollen morphology, Scanning electron microscopy, Cluster analysis |
| 基金项目:四川省科技计划(2021YFYZ0023、2020ZYD042)、四川省省级科研院所基本科研业务费(2021JDKY0023)资助 |
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| 中文摘要: |
| 为了解猕猴桃属植物间的亲缘关系,以41份不同猕猴桃花粉为供试材料,采用扫描电镜对猕猴桃花粉进行形态学观察和分析,选取6个性状指标进行测量,根据观测结果对供试材料进行聚类分析。结果表明：供试花粉均为单粒花粉,均以单粒、等极和辐射对称形式存在。极轴长度范围在21.50～33.40μm之间,赤道轴长度范围在9.87～16.24μm之间,萌发沟长度范围在17.53～29.08μm之间,萌发沟脊宽度范围在0.47～2.03μm之间,花粉粒大小(极轴×赤道轴))范围在218.68～543.18μm2之间,极轴长度与赤道轴长度比值范围为1.36～2.26,花粉形状为超长球形或长球形,萌发孔类型均为三孔沟,极面观为三裂圆形,均为N3P4C5型。外壁纹饰为波纹状、颗粒状、疣状三种类型,占样品总数的比例分别为63.41%、19.51%、17.08%。在遗传距离为7.5时,供试材料可以分为3个类群,在遗传距离为1.5时,供试材料可以分为6个类群。 |
| 英文摘要: |
| In order to understand the kinship between kiwifruit genera, forty-one different kiwifruit pollens were used as test materials,and the morphological observation of kiwifruit pollens were performed by scanning electron microscopy. Six trait indicators were selected for measurement, and then Q-clustering analysis was performed on the test materials based on the observation results. The results showed that the test pollen were all single-grain pollen, and all existed in single-grain, isopolar and radially symmetric forms. the polar axis lengths ranged from 21.50 to 33.40 μm, the equatorial axis lengths ranged from 9.87 to 16.24 μm, the germination furrow lengths ranged from 17.53 to 29.08 μm, the germination furrow ridge widths ranged from 0.47 to 2.03 μm, pollen grain size (polar axis × equatorial axis) ranged from 218.68 to 543.18 μm2, the ratio of polar axis length to equatorial axis length ranged from 1.36 to 2.26, The shape of the pollens was perprolate or prolate, and the germination pore types were all triple pore grooves.The polar surface view was triple cleft round, all of which were N3P4C5 type. The three types of outer wall ornamentation were corrugated, granular and warty, accounting for 63.41%, 19.51% and 17.08% of the total number of samples, respectively. At a genetic distance of 7.5, the test material can be divided into 3 taxa. At a genetic distance of 1.5, the test material could be divided into 6 taxa. |
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