| 张洁茹,杨航云,甘崧佑,董文娟,曾教科,向妙莲,陈金印,陈明.脐橙采后草酸浸泡处理抑制指状青霉侵染的效应[J].中国南方果树,2024,53(3): |
| 脐橙采后草酸浸泡处理抑制指状青霉侵染的效应 |
| Inhibitory Effect of Oxalic Acid Soaking Treatment on Penicillium Digitatum Infection In Postharvest Navel Oranges |
| 投稿时间：2023-07-25 修订日期：2023-08-24 |
| DOI：10.13938/j.issn.1007-1431.20230342 |
| 中文关键词: 脐橙 绿霉病 草酸 诱导抗性 抗氧化 |
| 英文关键词:Navel orange Green mold Oxalic acid Induced resistance Antioxidation |
| 基金项目:国家自然科学基金（32260793）；江西省柑橘产业技术体系（JXARS-07） |
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| 中文摘要: |
| 为研究外源草酸（Oxalic acid，OA）对脐橙果实抗采后绿霉病的诱导效应和抗氧化能力之间的关系，并筛选出外源草酸处理的适宜浓度。以‘龙回红’脐橙果实为试验材料，使用0（清水对照）、12.5、25.0、50.0 mmol·L-1草酸浸泡10分钟后接种指状青霉（Penicillium digitatum），通过分析病斑直径筛选出外源草酸处理的最适浓度，并测定总酚和类黄酮含量、抗氧化酶活性和丙二醛（Malonaldehyde，MDA）含量及其动态变化，探讨外源草酸诱导脐橙抗采后绿霉病的作用机制。结果表明，12.5、25.0、50.0 mmol·L-1草酸处理诱导脐橙抗绿霉病的诱导效果分别为16.26%、25.22%和17.36%，25.0 mmol·L-1草酸处理诱导效果显著高于另外两组。25.0 mmol·L-1草酸处理可以显著提高果实抗坏血酸过氧化物酶（Ascorbate peroxidase，APX）、过氧化物酶（Peroxidase，POD）、多酚氧化酶（Polyphenol oxidase，PPO）的活性，增加总酚和类黄酮的积累，提高自由基清除能力，抑制MDA的积累，从而减轻脐橙绿霉病的发生。25.0 mmol·L-1草酸处理提高了脐橙对采后绿霉病的抗性，可能与草酸诱导提高脐橙抗氧化能力密切相关。 |
| 英文摘要: |
| The study was to investigated the relationship between the induction effect of exogenous oxalic acid (OA) on the resistance to postharvest green mold and antioxidant capacity of navel orange fruits, then confirmed the appropriate concentration of exogenous OA treatment. ‘Longhuihong’ navel orange fruits were used as the experimental materials and soaked with 0 (Water treatment as control), 12.5, 25.0 and 50.0 mmol·L-1 OA for 10 min before Penicillium digitatum was inoculated in the fruits. The optimum concentration of exogenous OA treatment was screened by analyzing the diameter of lesions. Then the total phenols and flavonoids content, antioxidant enzymes activities and malonaldehyde (MDA) content and their dynamic changes were determined to analyzed the mechanism of exogenous OA induced the resistance against postharvest green mold in navel orange. The results showed that the induction effect of 12.5,25.0 and 50.0 mmol · L-1 OA treatment groups on the resistance of navel orange to green mold were 16.26 %, 25.22 % and 17.36 %, respectively. The induction effect of 25.0 mmol · L-1 OA treatment group was significantly higher than that of the other two groups. 25.0 mmol·L-1 OA treatment significantly increased the activities of ascorbate peroxidase (APX), peroxidase (POD) and polyphenol oxidase (PPO), promoted the accumulation of total phenols and flavonoids, improved the ability of free radical scavenging, inhibited the accumulation of MDA, then reduced the occurrence of green mold of navel orange. 25.0 mmol·L-1OA treatment increased the resistance of navel orange to postharvest green mold, which may be closely related to the increase of antioxidant capacity of navel orange induced by OA. |
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