| 孙新菊.转录组分析‘翠冠’梨种子层积过程中涉及ABA通路的关键基因[J].中国南方果树,2024,53(4): |
| 转录组分析‘翠冠’梨种子层积过程中涉及ABA通路的关键基因 |
| Transcriptome analysis reveals key genes associated with ABA pathway during the stratification process of ‘Cuiguan’ pear seed |
| 投稿时间：2023-11-01 修订日期：2023-11-20 |
| DOI： |
| 中文关键词: 梨 种子 脱落酸 转录组 |
| 英文关键词:Pear Seed ABA Transcriptome |
| 基金项目:2020年江苏省教育科学“十三五”规划青年专项重点资助项目阶段性研究成果（C-a/2020/01/14） |
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| 中文摘要: |
| 种子休眠与萌发受到内源激素和外界环境等多种因素的精确调控。生产上常采用低温沙藏层积处理的方式打破梨种子休眠进程，促进种子萌发。为解析梨种子休眠解除的分子机制，以‘翠冠’梨为研究对象，通过清水浸泡6小时梨种子，再低温沙藏处理60天。对该过程17个时期梨种子ABA含量进行测定，结果显示在种子浸泡过程和低温沙藏7天阶段，ABA含量整体呈现下降的趋势，随后趋于稳定。选取其中7个关键时期样品进行转录组测序，GO分析结果显示差异基因被分配到40个功能组，KEGG分析结果显示差异基因主要富集在核糖体、多样化环境中微生物代谢、碳代谢、辅因子的生物合成和氨基酸的生物合成通路。通过转录组和ABA含量数据构建共表达网络，结果显示11个基因（Pbr009501，Pbr016256，Pbr011612，Pbr033750，Pbr015393，Pbr000885，Pbr037755，Pbr039726，Pbr020341，Pbr003677和Pbr015873）与ABA含量相关性极高，相关性系数达到0.91-0.96。该研究结果为挖掘梨种子休眠和萌发过程调控ABA水平的关键基因提供了重要信息。 |
| 英文摘要: |
| Seed dormancy and germination are precisely regulated by various factors such as endogenous hormones and external environment. Low temperature stratification treatment is often used in production to break the dormancy process and promote germination of pear seeds. To analyze the molecular mechanism of dormancy release in pear seeds, we soak the ‘Cuiguan’ pear seeds in clean water for 6 hours and then low-temperature store them in sand for 60 days. The ABA content of pear seeds during 17 stages of this process was detected. The ABA content showed a decreasing trend during the seed soaking process and 7 days of low-temperature sand storage. After that, with the extension of sand storage time, the ABA content remained stable. Samples from seven key period were selected for RNA sequencing. The result of GO analysis showed that differentially expressed genes were assigned to 40 functional groups. The result of KEGG analysis showed that differentially expressed genes were mainly enriched in five pathways, including Ribosome, Microbial metabolism in diverse environments, Carbon metabolism, Biosynthesis of cofactors, and Biosynthesis of amino acids. A co-expression network was constructed using transcriptome and ABA content data. The result showed that 11 genes (Pbr009501, Pbr016256, Pbr011612, Pbr033750, Pbr015393, Pbr000885, Pbr037755, Pbr039726, Pbr020341, Pbr003677 and Pbr015873) were highly correlated with ABA content, with correlation coefficients ranging from 0.91 to 0.96. These results provide important information for exploring the key genes that regulate ABA levels during pear seed dormancy and germination processes. |
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