| 郑一羽,王仕玉,罗富寻,苏俊,何英云,张勤.‘茄梨’HY5基因的克隆及生物信息学分析[J].中国南方果树,2025,54(1): |
| ‘茄梨’HY5基因的克隆及生物信息学分析 |
| The Cloning and Bioinformatics Analysis of HY5 Gene of |
| 投稿时间：2023-11-29 修订日期：2024-01-27 |
| DOI：10.13938/j.issn.1007-1431.20230583 |
| 中文关键词: 茄梨 HY5 基因克隆 序列分析 |
| 英文关键词:Clapp’s Favorite HY5 Gene clone Sequence analysis |
| 基金项目:国家自然科学基金地区科学基金（编号:32260745 ），国家自然科学基金地区科学基金(编号:31760562)，云南省重大科技专项计划（编号:202302AE090005） |
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| 中文摘要: |
| 开展‘茄梨’HY5基因克隆及生物信息学分析,以期为基因功能的研究奠定基础。本试验以‘茄梨’为试材,提取mRNA,反转录因cDNA,扩增全长序列并进行生物信息学分析。结果显示：‘茄梨’HY5基因基因全长为495bp,编码164个氨基酸,其分子量为17.90KD,HY5为亲水蛋白,具有bZIP结构域；与云南红梨、白梨等蔷薇科植物的HY5基因具有高度的同源性,与中国白梨等HY5编码蛋白相似性为高达99.8%,表明其与梨属其他植物的HY5基因具有相同的功能。 |
| 英文摘要: |
| Cloning and bioinformatics analysis of HY5 gene of " Clapp’s Favorite " were carried out in order to lay a foundation for the study of gene function. In this experiment, we extracted mRNA, reverse transcription cDNA, amplified full-length sequence and analyzed bioinformatics of " Clapp’s Favorite ". The results showed that the total length of HY5 gene was 495bp, encoding 164 amino acids, and its molecular weight was 17.90KD. HY5 was a hydrophilic protein with bZIP domain. The HY5 gene has a high homology with the HY5 gene of Yunnan red pear, white pear and other rosaceae, and the similarity of HY5 encoding protein with the Chinese white pear is as high as 99.8%, indicating that it has the same function with the HY5 gene of other plants in the genus pear. |
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