| 李玉玲,王勇,SULAIMAN Aizezi,孙锋,伍国红.几种生长调节剂对‘昆香无核’胚挽救效果的影响[J].中国南方果树,2025,54(1): |
| 几种生长调节剂对‘昆香无核’胚挽救效果的影响 |
| Effects of several growth regulators on the embryo rescue effect of Kunxiang Wuhe grape |
| 投稿时间：2023-11-30 修订日期：2023-11-30 |
| DOI：10.13938/j.issn.1007-1431.20230586 |
| 中文关键词: ‘昆香无核’ 成苗率 花前 植物生长调节剂 |
| 英文关键词:Kunxiang Wuhe Percentage of plantlets Before flowers growth regulators |
| 基金项目:新疆维吾尔自治区自然科学(2022D01 A119)；财政部和农业农村部：国家现代农业产业技术体系资助(CARS-29-yc-7)。 |
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| 中文摘要: |
| 采用不同生长素类和细胞分裂素类生长调节剂对‘昆香无核’葡萄花前 2 w进行不同浓度的处理, 对果实的单果胚珠数、胚珠重量从35d开始每隔5d统计一次,同时以 ER 固液双相培养基作为胚发育培养基,WPM 固体培养基作为胚萌发培养基进行胚挽救,研究花前植物生长调节剂的施用对‘昆香无核’胚挽救效果的影响。结果表明BA、IBA、KT、CCC 不同浓度的处理都能使‘昆香无核’胚珠数增多; 用10 mg/L BA花前处理,种子大小指数为72.9%, 为最大, 而 5 mg/L CPPU 花前处理,种子大小指数为48.8%, 为最小；BA 5 mg/L 处理单果的平均种子数 2.9粒,为最多,而 IAA处理单果的平均种子数位1.0粒,为最少。花前BA5 mg/L 和10 mg/L 处理,IBA10 mg/L 和20 mg/L处理花穗,取果实进行胚珠培养后均可以提高‘昆香无核’的剥胚率和成苗率,以IBA20 mg/L成苗率最高达到13.6；花前喷施BA和IBA可以延缓胚珠败育5d以上。结果表明, 生长素类和细胞分裂素类生长调节剂可以提高无核葡萄胚挽救效率, 可以延缓胚珠败育,从而提高无核葡萄育种效率。 |
| 英文摘要: |
| Different concentrations of auxin and cytokinin growth regulators were used to treat the two weeks before flowering of Kunxiang seedless grape. The number of ovules per fruit and the weight of ovules were counted every 5 days from 35 days. At the same time, ER solid-liquid two-phase medium was used as embryo development medium, WPM solid medium was used as embryo germination medium for embryo rescue, and the effect of pre-anthesis plant growth regulator on the rescue effect of Kunxiang seedless embryo was studied. The results showed that different concentrations of BA, IBA, KT and CCC could increase the number of seedless ovules. With 10 mg / L BA pre-anthesis treatment, the seed size index was 72.9 %, which was the largest, while 5 mg / L CPPU pre-anthesis treatment, the seed size index was 48.8 %, which was the smallest. The average seed number of single fruit treated with BA 5 mg / L was 2.9, and the average seed number of single fruit treated with IAA was 1.0. The ovules produced by BA 5 mg / L and 10 mg / L treatment before flowering, IBA 10 mg / L and 20 mg / L treatment could improve the embryo stripping rate and seedling rate of kunxiang seedless. Spraying BA and IBA before anthesis could delay ovule abortion for more than 5 days. The results showed that auxin and cytokinin growth regulators could improve the seedling rate of seedless grape, delay ovule abortion and improve the breeding efficiency of seedless grape. |
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