| 王金星,王中华,杨青松,阚家亮,王宏,李晓刚.干旱胁迫下杜梨叶片差异表达mRNA的转录组分析[J].中国南方果树,2025,54(1): |
| 干旱胁迫下杜梨叶片差异表达mRNA的转录组分析 |
| Keywords: Pyrus betulifolia Bunge; Drought stress; Transcriptome; Differentially expressed genes |
| 投稿时间：2023-12-05 修订日期：2024-04-03 |
| DOI： |
| 中文关键词: 杜梨 干旱胁迫 转录组 差异表达基因 |
| 英文关键词:Pyrus betulifolia Bunge Drought stress Transcriptome Differentially expressed genes |
| 基金项目:江苏省农业科技自主创新资金项目[CX(23)1011] |
|
| 摘要点击次数: 106 |
| 全文下载次数: 0 |
| 中文摘要: |
| 干旱严重影响梨的生产,梨的主要砧木杜梨(Pyrus betulifolia Bunge)有抗旱的特点,挖掘和鉴定该基因资源对提高梨树抗旱能力具重要意义。本试验利用RNA-seq测序技术,以PEG干旱胁迫后CK、24h和48h的杜梨叶片为材料构建mRNA文库,经测序最终获得854,138,996bp个clean reads,和对照相比胁迫后24h、48h差异基因分别有3,660、2,078个,胁迫48h和24h相比差异表达基因共有2,212个。GO富集分析表明,差异表达基因主要富集在光合作用、有机环状化合物合成、信号识别、钙离子结合、氧化还原酶活性。KEGG分析表明,主要差异的代谢通路为果糖和甘露糖代谢、类黄酮生物合成、植物激素信号转导。光合固碳作用、碳代谢。经RT-qPCR验证,证实RNA-seq的数据可靠。 |
| 英文摘要: |
| Drought seriously affects the production of pears. The main rootstock of pears, Pyrus betulifolia Bunge has drought resistance characteristics. Exploring and identifying these gene resource is significance for improving the drought resistance ability of pear trees. This experiment utilized RNA seq sequencing technology to construct mRNA libraries from pear leaves treated with PEG drought stress at CK, 24, and 48 hours. After sequencing, 854,138,996 bp clean reads were obtained, with 3,660 and 2,078 differentially expressed genes compared to the control at 24 and 48 hours after stress, and a total of 2,212 differentially expressed genes compared with 48h VS 24h. GO enrichment analysis showed that differentially expressed genes were mainly enriched in photosynthesis, synthesis of organic cyclic compounds, signal recognition, calcium ion binding, and oxidoreductase activity. KEGG analysis shows that the main metabolic pathways are fructose and mannose metabolism, flavonoid biosynthesis, and plant hormone signal transduction, Photosynthetic carbon sequestration and carbon metabolism. After RT-qPCR validation, it was confirmed that the data of RNA seq is reliable. |
| 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
