| 谢建军,侯颖烨,赵菊鹏,荣晓东.检疫处理条件下荔枝中异菌脲残留量及降解动态研究[J].中国南方果树,2025,54(1): |
| 检疫处理条件下荔枝中异菌脲残留量及降解动态研究 |
| Determination of iprodione residue and degradation dynamics in litchi under quarantine treatment |
| 投稿时间：2023-12-12 修订日期：2024-02-26 |
| DOI：10.13938/j.issn.1007-1431.20230608 |
| 中文关键词: 荔枝 检疫处理 异菌脲 残留 降解动态 |
| 英文关键词:litchi quarantine treatment iprodione residue degradation dynamics |
| 基金项目:国家海关总署科技计划项目鲜荔枝检疫除害和绿色保鲜技术集成及应用示范(2023HK046),广州市科技重点研发项目荔枝高品质保鲜技术及装备研发与应用(2023b01j2001-6)项目资助。 |
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| 中文摘要: |
| 本文通过液质联用方法在特定检疫处理条件下研究荔枝中异菌脲残留含量及其降解动态。采用液相色谱-质谱联用仪检测荔枝中异菌脲残留含量，荔枝样品经乙腈提取，氯化钠盐析分层，Ｎ-丙基乙二胺（PSA）净化去除杂质，以多反应监测模式（MRM）测定，外标法定量。试验结果表明，在0.01~0.25 mg/kg范围内，异菌脲的质量浓度与对应的峰面积间呈线性关系，在添加水平为0.05 mg/kg、0.50 mg/kg和1.0 mg/kg下，荔枝中异菌脲的回收率为86.6%～98.4%，相对标准偏差为3.6%~8.3%，检出限为0.01 mg/kg。经异菌脲保鲜剂处理的3个荔枝品种桂味、淮枝、井岗红糯中异菌脲残留的降解符合一级动态方程Ct= 6.3e-0.067T、Ct= 5.9e-0.063T和Ct= 3.38e-0.05T，相关系数分别为0.9413、0.9656和0.8666，半衰期分别为11.36 d、11.95 d和15.75 d。试验发现，经异菌脲保鲜剂处理的荔枝在低温0.99℃、黑暗、密闭的检疫处理条件下，35 d后荔枝中异菌脲残留降解率都达到85.80%以上。本文可指导我国荔枝出口企业合理使用异菌脲，选择适当初始浓度的异菌脲处理荔枝既可以达到保鲜的目的，同时也可以达到检疫除害的要求，其残留量也符合对方国家的残留限量规定。 |
| 英文摘要: |
| For analyzing the residue and degradation dynamics of iprodione in litchi under specific quarantine conditions, the residue of iprodione in litchi was detected by high-pressure liquid chromatography-mass spectrometry (HPLC-MS/MS). Litchi samples were extracted by acetonitrile and salted out with sodium chloride. The extracts were purified by n-propyl ethylenediamine (PSA), detected by MRM mode, and quantified by external standard method. The results showed that there was a good linear relationship between the mass concentration and peak area in the range of 0.01~0.25 mg/kg. Under the spiked levels of 0.05 mg/kg, 0.50 mg/kg and 1.0 mg/kg, the recoveries were 86.6%～98.4% and the relative standard deviations were 3.6%~8.3% with the limit of detection (LOD) 0.01 mg/kg of iprodione in litchi. The degradation of iprodione in Guiwei, Huaizhi and Jinggang Hongnuo were in accordance with the first-order dynamic equation Ct= 6.3e-0.067T、Ct= 5.9e-0.063T and Ct= 3.38e-0.05T, while the correlation coefficients were 0.9413, 0.9656 and 0.8666, respectively. The half-lives of iprodione were 11.36 d, 11.95 d and 15.75 d. Under the condition of low temperature 0.99℃, darkness and in the fridge, the degradation rate of iprodione in litchi reached more than 85.80% after 35 days. This study provided a guidance for litchi export enterprises to use an appropriate initial concentration of iprodione as a litchi preservative in a reasonable manner with the purpose of preservation, meeting the requirements of quarantine, and complying with the residue limit regulations of the import countries. |
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