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朱晓菲,何程相,潘远智,许艺.核桃组培快繁体系的建立[J].中国南方果树,2023,52(1):
核桃组培快繁体系的建立
Establishment of Tissue Culture and Rapid Propagation System of Juglans regia
投稿时间:2021-11-23  修订日期:2021-11-23
DOI:10.13938/j.issn.1007-1431.20210731
中文关键词:  核桃  南核1号  组培  快繁体系
英文关键词:juglans regia  nanhe1  tissue culture  rapid propagation
基金项目:
作者单位E-mail
朱晓菲 成都市郫都区战旗农业发展有限公司 77483370@qq.com 
何程相* 四川天艺优境环境科技有限公司 525136129@qq.com 
潘远智 四川农业大学风景园林学院 scpyzls@163.com 
许艺 四川省农业科学院园艺研究所 763553664@qq.com 
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中文摘要:
      为建立‘南核1号’核桃组培快繁体系,以带芽茎段为外植体,开展外植体采集时间、初代诱导、继代增殖和生根培养各阶段试验。结果表明,全年中4-5月为最佳外植体采集时间;最佳诱导培养基为DKW+6-BA 1.0 mg/L+NAA 0.1mg/L,诱导率为66.7%;最佳增殖培养基为DKW+6-BA 1.0 mg/L+IBA 0.01mg/L,增殖率为63.3%,增殖系数为3.83;生根采用二步诱导生根法,先在附加IBA 6.0mg/L的1/2DKW中暗培养,再转移到1/4DKW中光培养,生根率为46.6%。
英文摘要:
      In order to establish the "Nanhe1" tissue culture and rapid propagation system,the stems were used as explants,the collection time,induction culture,prolifration culture,rooting culture were studied.The results showed that best collection time was 4-5 months;the best medium for bud induction was DKW+6-BA 1.0 mg/L+NAA 0.1mg/L, the induction rate was 66.7%;the best medium for proliferation was DKW+6-BA 1.0 mg/L+IBA 0.01mg/L,the proliferation rate was 63.3%,the average proliferation coefficient was 3.83;Using two-step induction rooting method, first dark culture in medium of 1/2DKW+ IBA 6.0mg/L,and then transferred to 1/4DKW light culture,the rooting rate was 46.6%.
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